RT Journal Article SR Electronic T1 In situ generation of RNA complexes for synthetic molecular strand displacement circuits in autonomous systems JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.07.15.204438 DO 10.1101/2020.07.15.204438 A1 Wooli Bae A1 Guy-Bart V. Stan A1 Thomas E. Ouldridge YR 2020 UL http://biorxiv.org/content/early/2020/07/15/2020.07.15.204438.abstract AB Synthetic molecular circuits implementing DNA or RNA strand-displacement reactions can be used to build complex systems such as molecular computers and feedback control systems. Despite recent advances, application of nucleic acid-based circuits in vivo remains challenging due to a lack of efficient methods to produce their essential components – multi-stranded complexes known as “gates” – in situ, i.e. in living cells or other autonomous systems. Here, we propose the use of naturally occurring self-cleaving ribozymes to cut a single-stranded RNA transcript into a gate complex of shorter strands, thereby opening new possibilities for the autonomous and continuous production of RNA strands in a stoichiometrically and structurally controlled way.Competing Interest StatementThe authors have declared no competing interest.