TY - JOUR T1 - Red blood cell tension controls <em>Plasmodium falciparum</em> invasion and protects against severe malaria in the Dantu blood group JF - bioRxiv DO - 10.1101/475442 SP - 475442 AU - Silvia N. Kariuki AU - Alejandro Marin-Menendez AU - Viola Introini AU - Benjamin J. Ravenhill AU - Yen-Chun Lin AU - Alex Macharia AU - Johnstone Makale AU - Metrine Tendwa AU - Wilfred Nyamu AU - Jurij Kotar AU - Manuela Carrasquilla AU - J. Alexandra Rowe AU - Kirk Rockett AU - Dominic Kwiatkowski AU - Michael P. Weekes AU - Pietro Cicuta AU - Thomas N. Williams AU - Julian C. Rayner Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/22/475442.abstract N2 - Malaria has had a major effect on the human genome, with many protective polymorphisms such as sickle cell trait having been selected to high frequencies in malaria endemic regions1, 2. Recently, we showed that a novel blood group variant, Dantu, provides 74% protection against all forms of severe malaria in homozygous individuals3-5. This is a similar degree of protection to sickle cell trait and considerably greater than the most advanced malaria vaccine, but until now the mechanism of protection has been unknown. In the current study, we demonstrate a significant impact of Dantu on the ability of Plasmodium falciparum merozoites to invade RBCs. The Dantu variant was associated with extensive changes to the RBC surface protein repertoire, but unexpectedly the malaria protective effect did not correlate with specific RBC-parasite receptor-ligand interactions. By following invasion using video microscopy, we found a strong link between RBC tension and parasite invasion and, even in non-Dantu RBCs, identified a tension threshold above which RBC invasion did not occur. Dantu RBCs had higher average tension, meaning that a higher proportion of Dantu RBCs could not be invaded. These findings not only provide an explanation for the protective effect of Dantu against severe malaria, but also provide fresh insights into the essential process of P. falciparum parasite invasion, and how invasion efficiency varies across the heterogenous populations of RBCs that are present both within and between individuals. ER -