PT  - JOURNAL ARTICLE
AU  - Dray, Nicolas
AU  - Mancini, Laure
AU  - Binshtok, Udi
AU  - Cheysson, Felix
AU  - Supatto, Willy
AU  - Mahou, Pierre
AU  - Bedu, Sébastien
AU  - Ortica, Sara
AU  - Krecsmarik, Monika
AU  - Herbert, Sébastien
AU  - Masson, Jean-Baptiste
AU  - Tinevez, Jean-Yves
AU  - Lang, Gabriel
AU  - Beaurepaire, Emmanuel
AU  - Sprinzak, David
AU  - Bally-Cuif, Laure
TI  - Dynamic spatiotemporal coordination of neural stem cell fate decisions through local feedback in the adult vertebrate brain
AID  - 10.1101/2020.07.15.205021
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.07.15.205021
4099  - http://biorxiv.org/content/early/2020/07/16/2020.07.15.205021.short
4100  - http://biorxiv.org/content/early/2020/07/16/2020.07.15.205021.full
AB  - Neural stem cell (NSC) populations persist in the adult vertebrate brain over a life time, and their homeostasis is controlled at the population level. The nature and properties of these coordination mechanisms remain unknown. Here we combine dynamic imaging of entire NSC populations in their in vivo niche over weeks, pharmacological manipulations, mathematical modeling and spatial statistics, and demonstrate that NSCs use spatiotemporally resolved local feedbacks to coordinate their decision to divide. These involve a Notch-mediated inhibition from transient neural progenitors, and a dispersion effect from dividing NSCs themselves, exerted with a delay of 9-12 days. Simulations from a stochastic NSC lattice model capturing these interactions demonstrate that they are linked by lineage progression and control the spatiotemporal distribution of output neurons. These results highlight how local and temporally delayed interactions occurring between brain germinal cells generate self-propagating dynamics that maintain NSC population homeostasis with specific spatiotemporal correlations.Competing Interest StatementThe authors have declared no competing interest.