PT  - JOURNAL ARTICLE
AU  - Xiaodan Wang
AU  - Zhenshuo Zhu
AU  - Xiaolong Wu
AU  - Hao Li
AU  - Tongtong Li
AU  - Qun Li
AU  - Peng Zhang
AU  - Leijie Li
AU  - Dongxue Che
AU  - Xia Xiao
AU  - Jinlian Hua
AU  - Mingzhi Liao
TI  - Landscape of RNA editing reveals new insights into the dynamic gene regulation of spermatogenesis based on integrated RNA-Seq
AID  - 10.1101/478206
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 478206
4099  - http://biorxiv.org/content/early/2018/11/24/478206.short
4100  - http://biorxiv.org/content/early/2018/11/24/478206.full
AB  - Spermatogenesis is an important physiological process associated with male infertility. But whether there are RNA editings (REs) and what’s the role of REs during the process are still unclear. In this study, we integrated published RNA-Seq datasets and established a landscape of REs during the development of mouse spermatogenesis. 7530 editing sites among all types of male germ cells were found, which enrich on some regions of chromosome, including chromosome 17 and both ends of chromosome Y. Totally, REs occur in 2012 genes during spermatogenesis, more than half of which harbor at two different sites of the same gene at least. We also found REs mainly occur in introns, coding regions (CDSs) and intergenic regions. Moreover, about half of the REs in CDSs can cause amino acids changes. Finally, based on our adult male Kunming mice, we verified that there is a non-synonymous A-to-I RNA editing site in Cog3 during spermatogenesis, which is conserved not only between species but also across tissues. In short, based on the power of integrating RNA-Seq datasets, we provided the landscape of REs and found their dynamic changes during mouse spermatogenesis. This research strategy is general for other types of sequencing datasets and biological problems.