RT Journal Article
SR Electronic
T1 Quantitative Analysis Reveals Reciprocal Regulations Underlying Recovery Dynamics of Thymocytes and Thymic Environment
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 478164
DO 10.1101/478164
A1 Kazumasa B. Kaneko
A1 Ryosuke Tateishi
A1 Takahisa Miyao
A1 Nobuko Akiyama
A1 Ryo Yokota
A1 Taishin Akiyama
A1 Tetsuya J. Kobayashi
YR 2018
UL http://biorxiv.org/content/early/2018/11/26/478164.abstract
AB Thymic crosstalk, a set of reciprocal regulations between thymocytes and thymic environment, is relevant for orchestrating the appropriate development of thymocytes as well as the recovery of the thymus from various exogenous insults. Nevertheless, the dynamic and regulatory aspects of the thymic crosstalk have not yet been clarified. In this work, we inferred the interactions shaping the thymic crosstalk and its resultant dynamics between the thymocytes and the thymic epithelial cells (TECs) by quantitative analysis and modelling of the recovery dynamics induced by irradiation. The analysis identified regulatory interactions consistent with the known molecular evidence and revealed their dynamic roles in the recovery process. Moreover, the analysis also predicted, and a subsequent experiment verified a new regulation of CD4+CD8+ double positive (DP) thymocytes, which temporarily increases their proliferation rate upon the decrease in their population size. Our model established the pivotal step towards a dynamic understanding of the thymic crosstalk as a regulatory network system.