RT Journal Article
SR Electronic
T1 Spatio-temporal organization of cell assemblies in Nucleus Reuniens during slow oscillations
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 474973
DO 10.1101/474973
A1 David Angulo-Garcia
A1 Maëva Ferraris
A1 Antoine Ghestem
A1 Christophe Bernard
A1 Pascale P Quilichini
YR 2018
UL http://biorxiv.org/content/early/2018/11/26/474973.abstract
AB The nucleus reuniens (NR) is an important anatomical and functional relay between the medial prefrontal cortex (mPFC) and the hippocampus (HPC). Whether the NR controls neuronal assemblies - a hallmark of information exchange between the HPC and mPFC for memory transfer/consolidation - is not known. Using simultaneous LFP and unit recordings in NR, HPC and mPFC in rats during slow oscillations under anesthesia, we identified a reliable sequential activation of NR neurons at the beginning of UP states, which preceded mPFC ones. NR sequences were spatially organized, from dorsal to ventral NR. Chemical inactivation of the NR disrupted mPFC sequences at the onset of UP states as well as HPC sequences present during sharp-wave ripples. We conclude that the NR contributes to the coordination and stabilization of mPFC and HPC neuronal sequences during slow oscillations, possibly via the early activation of its own sequences.This work was supported by grants from the FRM (Fondation Recherche Médicale FDT201805005246 to M. F.). D.A-G was received support by the A*MIDEX grant (No. ANR-11-IDEX-0001-02). We thank Wesley Clawson for proofreading this manuscript.Author Contributions:M.F. and P.P.Q. designed the experiments. M.F. and P.P.Q. performed the experiments. A.G. assisted in performing experiments. D.A.G., M.F. and P.P.Q. interpreted the results and performed the analysis. All the authors reviewed and edited the manuscript.