TY - JOUR T1 - Quantitative mapping of membrane nanoenvironments through single-molecule imaging of solvatochromic probes JF - bioRxiv DO - 10.1101/2020.07.19.209908 SP - 2020.07.19.209908 AU - Daniel J. Nieves AU - Dylan M. Owen Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/07/19/2020.07.19.209908.abstract N2 - Environmentally-sensitive fluorophores report on their local biochemical or biophysical environments through changes in their emission. We combine the solvatochromic probe di-4-ANEPPDHQ with multi-channel SMLM and quantitative analysis of the resulting marked point patterns to map biophysical environments in the mammalian cell membrane. We show that plasma membrane properties can be mapped with nanoscale resolution, and that partitioning between ordered and disordered regions is observed on length scales below 300 nm.Competing Interest StatementThe authors have declared no competing interest. ER -