RT Journal Article
SR Electronic
T1 Acute and chronic stage adaptations of vascular architecture and cerebral blood flow in a mouse model of TBI
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 479626
DO 10.1101/479626
A1 Joe Steinman
A1 Lindsay S. Cahill
A1 Margaret M. Koletar
A1 Bojana Stefanovic
A1 John G. Sled
YR 2018
UL http://biorxiv.org/content/early/2018/11/27/479626.abstract
AB The 3D organization of cerebral blood vessels determines the overall capacity of the cerebral circulation to meet the metabolic requirements of the brain. This study used Arterial Spin Labeling (ASL) MRI with a hypercapnic challenge and ex vivo Serial Two-Photon Tomography (STPT) to examine the relationship between blood flow and 3D microvascular structure following traumatic brain injury (TBI) in a mouse. Mice were exposed to a controlled cortical impact TBI and allowed to recover for either 1 day or 4 weeks. At each time point, ASL MRI was performed to quantify cerebral perfusion and the brain vasculature was imaged in 3D with STPT. Registration of ASL to STPT enabled flow changes to be related to the underlying microvascular structure in each ASL voxel. Hypoperfusion under rest and hypercapnia was observed both 1 day and 4 weeks post-TBI. Vessel density and vascular volume were reduced 1 day post-TBI, recovering by 4 weeks; however, the reorganized vasculature at the latter time point possessed an abnormal radial pattern. Our findings demonstrate functionally significant long-term changes in the vascular architecture following injury and illustrate why metrics beyond traditional measures of vessel density are required to understand the impact of vascular structure on function.