TY - JOUR T1 - The human secretome – the proteins secreted from human cells JF - bioRxiv DO - 10.1101/465815 SP - 465815 AU - Mathias Uhlen AU - Hanna Tegel AU - Åsa Sivertsson AU - Chih-Chung Kuo AU - Jahir M. Gutierrez AU - Nathan E. Lewis AU - Björn Forsström AU - Melanie Dannemeyer AU - Linn Fagerberg AU - Magdalena Malm AU - Helian Vunk AU - Fredrik Edfors AU - Andreas Hober AU - Evelina Sjöstedt AU - David Kotol AU - Jan Mulder AU - Adil Mardinoglu AU - Jochen M. Schwenk AU - Peter Nilsson AU - Martin Zwahlen AU - Jenny Ottosson Takanen AU - Kalle von Feilitzen AU - Charlotte Stadler AU - Cecilia Lindskog AU - Fredrik Ponten AU - Jens Nielsen AU - Bernhard O. Palsson AU - Anna-Luisa Volk AU - Magnus Lundqvist AU - Anna Berling AU - Anne-Sophie Svensson AU - Sara Kanje AU - Henric Enstedt AU - Delaram Afshari AU - Siri Ekblad AU - Julia Scheffel AU - Borbala Katona AU - Jimmy Vuu AU - Emil Lindström AU - LanLan Xu AU - Roxana Mihai AU - Lucas Bremer AU - Malin Westin AU - Muna Muse AU - Lorenz M. Mayr AU - Sinead Knight AU - Sven Göpel AU - Rick Davies AU - Paul Varley AU - Diane Hatton AU - Ray Fields AU - Bjørn G. Voldborg AU - Johan Rockberg AU - Lovisa Holmberg Schiavone AU - Sophia Hober Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/27/465815.abstract N2 - The proteins secreted by human tissues (the secretome) are important for the basic understanding of human biology, but also for identification of potential targets for future diagnosis and therapy. Here, we present an annotation of all predicted secreted proteins (n=2,623) with information about their spatial distribution in the human body. A high-throughput mammalian cell factory was established to create a resource of recombinant full-length proteins. This resource was used for phenotypic assays involving β-cell dedifferentiation and for development of targeted proteomics assays. A comparison between host cells, including omics analysis, shows that many of the proteins that failed to be generated in CHO cells could be rescued in human HEK293 cells. In conclusion, the human secretome has been mapped and characterized to facilitate further exploration of the human secretome. ER -