RT Journal Article SR Electronic T1 Evidence of exposure to SARS-CoV-2 in cats and dogs from households in Italy JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.07.21.214346 DO 10.1101/2020.07.21.214346 A1 Patterson, E.I. A1 Elia, G. A1 Grassi, A. A1 Giordano, A. A1 Desario, C. A1 Medardo, M. A1 Smith, S.L. A1 Anderson, E.R. A1 Prince, T. A1 Patterson, G.T. A1 Lorusso, E. A1 Lucente, M.S. A1 Lanave, G. A1 Lauzi, S. A1 Bonfanti, U. A1 Stranieri, A. A1 Martella, V. A1 Basano, F. Solari A1 Barrs, V.R. A1 Radford, A.D. A1 Agrimi, U. A1 Hughes, G. L. A1 Paltrinieri, S. A1 Decaro, N. YR 2020 UL http://biorxiv.org/content/early/2020/07/23/2020.07.21.214346.abstract AB SARS-CoV-2 originated in animals and is now easily transmitted between people. Sporadic detection of natural cases in animals alongside successful experimental infections of pets, such as cats, ferrets and dogs, raises questions about the susceptibility of animals under natural conditions of pet ownership. Here we report a large-scale study to assess SARS-CoV-2 infection in 817 companion animals living in northern Italy, sampled at a time of frequent human infection. No animals tested PCR positive. However, 3.4% of dogs and 3.9% of cats had measurable SARS-CoV-2 neutralizing antibody titers, with dogs from COVID-19 positive households being significantly more likely to test positive than those from COVID-19 negative households. Understanding risk factors associated with this and their potential to infect other species requires urgent investigation.One Sentence Summary SARS-CoV-2 antibodies in pets from Italy.Competing Interest StatementThe authors have declared no competing interest.