RT Journal Article
SR Electronic
T1 The BMP antagonist <em>Gremlin1</em> contributes to the development of cortical excitatory neurons, motor balance and fear responses
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.24.219394
DO 10.1101/2020.07.24.219394
A1 Mari Ichinose
A1 Nobumi Suzuki
A1 Tongtong Wang
A1 Hiroki Kobayashi
A1 Laura Vrbanac
A1 Jia Q Ng
A1 Josephine A Wright
A1 Tamsin R M Lannagan
A1 Krystyna A Gieniec
A1 Martin Lewis
A1 Ryota Ando
A1 Atsushi Enomoto
A1 Simon Koblar
A1 Paul Thomas
A1 Daniel L Worthley
A1 Susan L Woods
YR 2020
UL http://biorxiv.org/content/early/2020/07/24/2020.07.24.219394.abstract
AB The role and modulators of bone morphogenetic protein (BMP) signaling pathway in later cortical patterning awaits careful mechanistic investigation. Here we show that the BMP antagonist, Grem1, marks a neuroprogenitor that gives rise to layer Ⅴ and Ⅵ glutamatergic neurons in the embryonic mouse brain. Lineage tracing of Grem1-expressing cells in the fetal brain was examined by administration of tamoxifen to pregnant Grem1creERT; Rosa26LSLTdtomato mice at 13.5 days post coitum. In addition, bulk mRNA seq analysis of differentially expressed transcripts between FACS sorted Grem1 positive and negative cells was performed. We also generated Grem1 conditional knockout mice (Emx1-Cre;Grem1flox/flox) in which the Grem1 gene was deleted specifically in the dorsal telencephalon. Grem1Emx1cKO animals had reduced cortical thickness, especially layers Ⅴ/Ⅵ and impaired motor balance and fear sensitivity compared to littermate controls. This study has revealed new roles for Grem1 in the structural and functional maturation of the developing cortex.Competing Interest StatementThe authors have declared no competing interest.