@article {Stewart224147, author = {Thomas A. Stewart and Cong Liang and Justin L. Cotney and James P. Noonan and Thomas J. Sanger and P. Wagner G{\"u}nter}, title = {Evidence against tetrapod-wide digit identities and for a limited frame shift in bird wings}, elocation-id = {224147}, year = {2018}, doi = {10.1101/224147}, publisher = {Cold Spring Harbor Laboratory}, abstract = {In crown group tetrapods, individual digits are homologized in relation to a pentadactyl ground plan. However, testing hypotheses of digit homology is challenging because it is unclear whether digits develop with distinct and conserved gene regulatory states. Here we show dramatic evolutionary dynamism in the gene expression profiles of digits, challenging the notion that five digit identities are conserved across amniotes. Transcriptomics of developing limbs shows diversity in the patterns of genetic differentiation of digits, although the anterior-most digit of the pentadactyl limb has a unique, conserved expression profile. Further, we identify a core set of transcription factors that are differentially expressed among the digits of amniote limbs; their spatial expression domains, however, vary between species. In light of these results, we reevaluate the frame shift hypothesis of avian wing evolution and conclude that only the identity of the anterior-most digit has shifted position, suggesting a 1,3,4 digit identity in the bird wing.}, URL = {https://www.biorxiv.org/content/early/2018/12/01/224147}, eprint = {https://www.biorxiv.org/content/early/2018/12/01/224147.full.pdf}, journal = {bioRxiv} }