PT  - JOURNAL ARTICLE
AU  - Daniel Sage
AU  - Thanh-An Pham
AU  - Hazen Babcock
AU  - Tomas Lukes
AU  - Thomas Pengo
AU  - Jerry Chao
AU  - Ramraj Velmurugan
AU  - Alex Herbert
AU  - Anurag Agrawal
AU  - Silvia Colabrese
AU  - Ann Wheeler
AU  - Anna Archetti
AU  - Bernd Rieger
AU  - Raimund Ober
AU  - Guy M. Hagen
AU  - Jean-Baptiste Sibarita
AU  - Jonas Ries
AU  - Ricardo Henriques
AU  - Michael Unser
AU  - Seamus Holden
TI  - Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software
AID  - 10.1101/362517
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 362517
4099  - http://biorxiv.org/content/early/2018/12/01/362517.1.short
4100  - http://biorxiv.org/content/early/2018/12/01/362517.1.full
AB  - With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities – 2D, astigmatic 3D, biplane 3D, and double helix 3D – and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.