PT  - JOURNAL ARTICLE
AU  - Alexandru Paraschiv
AU  - Thibaut J. Lagny
AU  - Evelyne Coudrier
AU  - Patricia Bassereau
AU  - Anđela Šarić
TI  - Influence of membrane-cortex linkers on the extrusion of membrane tubes
AID  - 10.1101/2020.07.28.224741
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.07.28.224741
4099  - http://biorxiv.org/content/early/2020/07/28/2020.07.28.224741.short
4100  - http://biorxiv.org/content/early/2020/07/28/2020.07.28.224741.full
AB  - The cell membrane is an inhomogeneous system composed of phospholipids, sterols and proteins that can be directly attached to underlying cytoskeleton. The linkers between the membrane and the cytoskeleton are believed to have a profound effect on the mechanical properties of the cell membrane and its ability to reshape. Here we investigate the role of membrane-cortex linkers on the extrusion of membrane tubes using computer simulations and experiments. In simulations we find that the force for tube extrusion has a non-linear dependence on the density of membrane-cortex attachments: at a wide range of low and intermediate densities of linkers the force is not significantly influenced by the presence of membrane linking proteins and resembles that of the bare membrane. For large concentrations of linkers however the force substantially increases compared to the bare membrane. In both cases the linkers provided membrane tubes with increased stability against coalescence. We then pulled tubes from HEK cells using optical-tweezers for varying expression levels of the membrane-cortex attachment protein Ezrin. In line with simulations, we observed that overexpression of Ezrin led to an increased extrusion force, while Ezrin depletion had negligible effect on the force. Our results shed new light on the importance of local effects in membrane reshaping at the nanoscopic scales.Competing Interest StatementThe authors have declared no competing interest.