RT Journal Article
SR Electronic
T1 Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathways
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 487264
DO 10.1101/487264
A1 Yen-Chung Lin
A1 Mai-Szu Wu
A1 Chang-Rong Chen
A1 Chang-Yu Chen
A1 Chang-Jui Chen
A1 Kuan-Chou Chen
A1 Chiung-Chi Peng
YR 2018
UL http://biorxiv.org/content/early/2018/12/04/487264.abstract
AB Lipid accumulation in renal cells has been implicated in the pathogenesis of obesity-related kidney disease, and lipotoxicity occurring in the kidney can be a surrogate marker for renal failure or renal fibrosis. Nifedipine-induced renal lipotoxicity has never been cited, although a few studies have shown that nifedipine inhibits lipogenesis via activation of the LKB1-AMPK pathway. Therefore, we utilized NRK52E cell models to examine this further. We pre-treated cells with varying concentrations of nifedipine (7.5, 15, or 30 μM) for 24 or 48 h prior to examining the activity of lipogenesis enzymes and lipotoxicity.Nifedipine was found to activate acetyl CoA synthetase, acetyl CoA carboxylase, long chain fatty acyl CoA elongase, ATP-citrate lyase, and HMG CoA reductase, suggesting elevated production of cholesterol, triacylglycerides, and phospholipids. Nifedipine exposure induced a vast accumulation of cytosolic free fatty acids (FFA) and stimulated the production of reactive oxygen species, upregulated CD36 and KIM-1 (kidney injury molecule-1) expression, inhibited p-AMPK activity, and triggered the transcription of SREBP-1/2 and lipin-1, underscoring the potential of nifedipine to induce lipotoxicity with renal damage.In the present study, we elucidated the mechanism of action of nifedipine that leads to renal lipotoxicity via the AMPK-SREBP-1/2 pathway. To our knowledge, this is the first report demonstrating nifedipine-induced lipid accumulation in the kidney.