PT - JOURNAL ARTICLE AU - Valentin Quiniou AU - Pierre Barennes AU - Federica Martina AU - Vanessa Mhanna AU - Helene Vantomme AU - Hang Phuong Pham AU - Mikhail Shugay AU - Adrien Six AU - Encarnita Mariotti-Ferrandiz AU - David Klatzmann TI - Human thymopoiesis selects unconventional CD8<sup>+</sup> α/β T cells that respond to multiple viruses AID - 10.1101/2020.07.27.223354 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.07.27.223354 4099 - http://biorxiv.org/content/early/2020/07/29/2020.07.27.223354.short 4100 - http://biorxiv.org/content/early/2020/07/29/2020.07.27.223354.full AB - T cell receptors (TCRs) are formed by stochastic gene rearrangements, theoretically generating ≫1019 sequences1. They are selected during thymopoiesis, which releases a repertoire of about 108 unique TCRs2,3 per individual. How evolution shaped a process that produces TCRs that would effectively respond to infectious agents is a central question of immunology. The paradigm is that a diverse enough repertoire of TCRs should always provide a proper, though rare, specificity for any given need. Expansion of such rare T cells would provide enough fighters for an efficacious immune response and enough antigen-experienced cells for memory3,4. We show here that thymopoiesis releases a large population of CD8+ T cells harbouring diverse α/βTCRs with innate-like properties. These TCRs (i) have high generation probabilities and a preferential usage of some V and J genes, (ii) are shared between individuals, (iii) are highly enriched for viral antigen recognition and (iv) have a fuzzy rather than tight specificity. In vitro, T cells expressing these TCRs bind to and are activated by multiple unrelated viral peptides; in vivo, they respond to vaccination and infection, being notably found in bronchoalveolar lavages of COVID-19 infected patients. Our results support an evolutionary selection of pleiospecific α/βTCRs for broad antiviral responses and heterologous immunity.Competing Interest StatementThe authors have declared no competing interest.