RT Journal Article
SR Electronic
T1 Systemic sterile induced-co-expression of IL-12 and IL-18 drives IFN-γ-dependent activation of microglia and recruitment of MHC-II-expressing inflammatory monocytes into the brain
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.30.228098
DO 10.1101/2020.07.30.228098
A1 Gaviglio, Emilia A.
A1 Peralta Ramos, Javier M.
A1 Arroyo, Daniela S.
A1 Bussi, Claudio
A1 Iribarren, Pablo
A1 Rodriguez-Galan, Maria C.
YR 2020
UL http://biorxiv.org/content/early/2020/07/30/2020.07.30.228098.abstract
AB The development of neuroinflammation, as well as the progression of several neurodegenerative diseases, has been associated with the activation and mobilization of the peripheral immune system due to systemic inflammation. However, the mechanism by which this occurs remains unclear. Herein, we addressed the effect of systemic, endotoxin-free induced-co-expression of IL-12 and IL-18 in the establishment of a novel cytokine-mediated model of neuroinflammation. Following peripheral hydrodynamic shear of IL-12 plus IL-18 cDNAs in C57BL/6 mice, we induced systemic and persistent level of IL-12, which in turn promoted the elevation of circulating pro-inflammatory cytokines TNF-α and IFN-γ, accompanied with splenomegaly. Moreover, even though we identified an increased gene expression of both TNF-α and IFN-γ in the brain, only TNF-α was shown to be dispensable, revealing an IFN-γ-dependent activation of microglia and the recruitment of leukocytes, particularly of highly activated inflammatory monocytes. Taken together, our results argue for a systemic cytokine-mediated establishment and development of neuroinflammation, having identified IFN-γ as a potential target for immunotherapy.Competing Interest StatementThe authors have declared no competing interest.