PT  - JOURNAL ARTICLE
AU  - Brian Joseph
AU  - Eric C. Lai
TI  - The Exon Junction Complex and intron removal prevents resplicing of mRNA
AID  - 10.1101/2020.07.31.231498
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.07.31.231498
4099  - http://biorxiv.org/content/early/2020/07/31/2020.07.31.231498.short
4100  - http://biorxiv.org/content/early/2020/07/31/2020.07.31.231498.full
AB  - Accurate splice site selection is critical for fruitful gene expression. Here, we demonstrate the Drosophila EJC suppresses hundreds of functional cryptic splice sites (SS), even though majority of these bear weak splicing motifs and appear incompetent. Mechanistically, the EJC directly conceals splicing elements through position-specific recruitment, preventing SS definition. We note that intron removal using strong, canonical SS yields AG|GU signatures at exon-exon junctions. Unexpectedly, we discover that scores of these minimal exon junction sequences are in fact EJC-suppressed 5’ and 3’ recursive SS, and that loss of EJC regulation from such transcripts triggers faulty mRNA resplicing. An important corollary is that intronless cDNA expression constructs from aforementioned targets yield high levels of unanticipated, truncated transcripts generated by resplicing. Consequently, we conclude the EJC has ancestral roles to defend transcriptome fidelity by (1) repressing illegitimate splice sites on pre-mRNAs, and (2) preventing inadvertent activation of such sites on spliced segments.