RT Journal Article
SR Electronic
T1 Structure of a type IV CRISPR-Cas effector complex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.31.231399
DO 10.1101/2020.07.31.231399
A1 Yi Zhou
A1 Jack P.K. Bravo
A1 Hannah N. Taylor
A1 Jurre Steens
A1 Ryan N. Jackson
A1 Raymond H.J. Staals
A1 David W. Taylor
YR 2020
UL http://biorxiv.org/content/early/2020/07/31/2020.07.31.231399.abstract
AB We reveal the structure of a type IV-B CRISPR effector (Csf) complex at 3.9 Å resolution using cryo-electron microscopy. The complex resembles the type III-A CRISPR Csm effector complex, but lacks subunits for RNA processing and target DNA cleavage, and is surprisingly assembled upon heterogeneous non-CRISPR RNA. These findings provide the first glimpse into the assembly and function of enigmatic type IV CRISPR systems.Competing Interest StatementThe authors have declared no competing interest.