RT Journal Article
SR Electronic
T1 Deregulation of VEGFR-2 and PGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 490144
DO 10.1101/490144
A1 Denner Santos dos Anjos
A1 Aline Fernandes Vital
A1 Patrícia de Faria Lainetti
A1 Antonio Fernando Leis-Filho
A1 Fabiola Dalmolin
A1 Fabiana Elias
A1 Sabryna Gouveia Calazans
A1 Carlos Eduardo Fonseca-Alves
YR 2018
UL http://biorxiv.org/content/early/2018/12/08/490144.abstract
AB Canine mammary tumors (CMT) are the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of VEGFR-2, PDGFR and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (N=11), tumors with lymph node metastasis (LNM) (N=10) and tumors with lung metastasis (LM) (N=5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression.