PT  - JOURNAL ARTICLE
AU  - Giantommaso Scarascia
AU  - Luca Fortunato
AU  - Yevhen Myshkevych
AU  - Hong Cheng
AU  - TorOve Leiknes
AU  - Pei-Ying Hong
TI  - UV and bacteriophages as a chemical-free approach for cleaning membranes from anaerobic bioreactors
AID  - 10.1101/2020.08.03.234450
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.08.03.234450
4099  - http://biorxiv.org/content/early/2020/08/03/2020.08.03.234450.short
4100  - http://biorxiv.org/content/early/2020/08/03/2020.08.03.234450.full
AB  - Anaerobic membrane bioreactor (AnMBR) for wastewater treatment has attracted much interest due to its efficacy in providing high quality effluent with minimal energy costs. However, membrane biofouling represents the main bottleneck for AnMBR because it diminishes flux and necessitates frequent replacement of membranes. In this study, we assessed the feasibility of combining bacteriophages and UV-C irradiation to provide a chemical-free approach to remove biofoulants on the membrane. The combination of bacteriophage and UV-C resulted in better log cells removal and twice higher extracellular polymeric substance (EPS) concentration reduction in mature biofoulants compared to UV-C. A reduction in the relative abundance of Acinetobacter spp. and selected gram-positive bacteria associated with the membrane biofilm was also achieved by the new cleaning approach. Microscopic analysis further revealed the formation of cavities in the biofilm due to bacteriophages and UV-C irradiation, which would be beneficial to maintain water flux through the membrane. When the combined treatment was further compared with the common chemical cleaning procedure, a similar reduction on the cell numbers was observed (1.4 log). However, combined treatment was less effective in removing EPS compared with chemical cleaning. These results suggest that the combination of UV-C and bacteriophage have an additive effect in biofouling reduction, representing a potential chemical-free method to remove reversible biofoulants on membrane fitted in an anaerobic membrane bioreactor.SIGNIFICANCE Anaerobic membrane bioreactors can achieve high quality effluent with a reduced energy consumption. However, biofouling represents the main bottleneck for membrane filtration efficiency. Biofouling is commonly reduced through chemical treatment. These agents are often detrimental for the environment and health safety due to the formation of toxic byproducts. Therefore, we present a new approach, based on the additive antifouling action of bacteriophages infection and UV-C irradiation, to reduce anaerobic membrane biofouling. This new strategy could potentially delay the occurrence of membrane fouling by removing the reversible fouling layers on membranes, in turn reducing the frequencies and amount of chemicals needed throughout the course of wastewater treatment.Competing Interest StatementThe authors have declared no competing interest.