PT - JOURNAL ARTICLE AU - Alexey Shipunov AU - Hye Ji Lee AU - Jinhee Choi AU - Kyle Pay AU - Sarah DeSpiegelaire AU - Aaron Floden TI - Not out of the box: phylogeny of the broadly sampled Buxaceae AID - 10.1101/2020.08.03.235267 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.08.03.235267 4099 - http://biorxiv.org/content/early/2020/08/04/2020.08.03.235267.short 4100 - http://biorxiv.org/content/early/2020/08/04/2020.08.03.235267.full AB - The Buxaceae constitute a morphologically diverse phylogenetic lineage of six genera, which includes about 140 species. The most well-known genera are Buxus, Sarcococca, and Pachysandra. Few species of woody Styloceras grow on mid-elevations in the Andes mountains region. Didymeles, with three species endemic to Madagascar, and the monotypic Haptanthus from Honduras, are the most unusual members of the group. The infra-familial classification of Buxaceae is controversial, and molecular data about many species, especially Old World, is still lacking. We used broad taxonomic sampling and molecular data from four chloroplast markers, and the nuclear ribosomal ITS to estimate their phylogeny. These data provide phylogenetic placements of 50 species and enabled better estimates of boundaries in Buxaceae. We described two subfamilies, two monotypic genera, two Buxus subgenera, and one new species of Didymeles from Madagascar.Competing Interest StatementThe authors have declared no competing interest.