RT Journal Article
SR Electronic
T1 Structure of a cytochrome-based bacterial nanowire
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 492645
DO 10.1101/492645
A1 Filman, David J.
A1 Marino, Stephen F.
A1 Ward, Joy E.
A1 Yang, Lu
A1 Mester, Zoltán
A1 Bullitt, Esther
A1 Lovley, Derek R.
A1 Strauss, Mike
YR 2018
UL http://biorxiv.org/content/early/2018/12/10/492645.abstract
AB Electrically conductive pili from Geobacter species, termed bacterial “nanowires”, are intensely studied for their biological significance and potential in the development of new materials. We have characterized a unique nanowire from conductive G. sulfurreducens pili preparations by cryo-electron microscopy composed solely of the c-type cytochrome OmcS. We present here, at 3.4 Å resolution, a novel structure of a cytochrome-based filament and discuss its possible role in long-range biological electron transport.Summary sentence Cryo-electron microscopy reveals the remarkable assembly of a c-type cytochrome into filaments comprising a heme-based bacterial nanowire.