PT  - JOURNAL ARTICLE
AU  - Surabhi Sonam
AU  - Sushant Bangru
AU  - Kimberly J. Perry
AU  - Auinash Kalsotra
AU  - Jonathan J. Henry
TI  - Single-cell analyses of the corneal epithelium: Unique cell types and gene expression profiles
AID  - 10.1101/2020.08.06.240036
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.08.06.240036
4099  - http://biorxiv.org/content/early/2020/08/06/2020.08.06.240036.short
4100  - http://biorxiv.org/content/early/2020/08/06/2020.08.06.240036.full
AB  - Corneal Epithelial Stem Cells (CESCs) and their proliferative progeny, the Transit Amplifying Cells (TACs), are responsible for homeostasis and maintaining corneal transparency. Owing to our limited knowledge of cell fates and gene activity within the cornea, the search for unique markers to identify and isolate these cells remains crucial for ocular surface reconstruction. We performed single-cell RNA sequencing of corneal epithelial cells from stage 49-51 Xenopus larvae. We identified five main clusters with distinct molecular signatures, which represent apical, basal and keratocyte cell types as well as two discrete proliferative cell types in the bi-layered epithelium. Our data reveal several novel genes expressed in corneal cells and spatiotemporal changes in gene expression during corneal differentiation. Through gene regulatory network analysis, we identified key developmental gene regulons, which guide these different cell states. Our study offers a detailed atlas of single-cell transcriptomes in the frog corneal epithelium. In future, this work will be useful to elucidate the function of novel genes in corneal homeostasis, wound healing and cornea regeneration, which includes lens regeneration in Xenopus.SUMMARY STATEMENT This study identifies cell types and transcriptional heterogeneity in the corneal epithelium that regulate its differentiation, and facilitates the search for corneal stem cell markers.