RT Journal Article
SR Electronic
T1 Development and characterization of infectious clones of two strains of Usutu virus
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.05.238543
DO 10.1101/2020.08.05.238543
A1 Tyler Bates
A1 Christina Chuong
A1 Seth A. Hawks
A1 Pallavi Rai
A1 Nisha K. Duggal
A1 James Weger-Lucarelli
YR 2020
UL http://biorxiv.org/content/early/2020/08/07/2020.08.05.238543.1.abstract
AB Usutu virus (USUV; genus Flavivirus; family Flaviviridae) is a mosquito-borne, positive-sense RNA virus that is currently causing significant die-offs in numerous bird species throughout Europe and has caused infections in humans. Currently, there are no molecular clones for USUV, hence, hindering studies on the pathogenesis and transmission of USUV. In this report, we demonstrate the development and characterization of infectious clones for two modern strains of USUV isolated from Europe and Africa. We show that the infectious clone-derived viruses replicated similarly to the parental strains in both mammalian and insect cells. Additionally, we observed similar levels of replication and pathogenesis in two mouse models. This reverse genetics system will aid the scientific community in studying and developing USUV infection, transmission, diagnostics, and vaccines.Competing Interest StatementThe authors have declared no competing interest.