PT  - JOURNAL ARTICLE
AU  - Chen, Wei
AU  - Kang, Tao
AU  - Yuan, Rongliang
AU  - Zhang, Yuyang
AU  - Xin, Siqi
AU  - Shao, Congwen
AU  - Jing, Shenrong
TI  - Immunogenicity and protective potency of Norovirus GII.17 virus-like particle-based vaccine
AID  - 10.1101/496190
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 496190
4099  - http://biorxiv.org/content/early/2018/12/13/496190.short
4100  - http://biorxiv.org/content/early/2018/12/13/496190.full
AB  - Noroviruses (NoVs) are a major cause of acute viral gastroenteritis in adults and children worldwide. Lacking of cell culture system and animals models that must be considered the virus like particles (VLPs) used as an effective vaccine development. In the present study, we investigated the expression of the major capsid protein (VP1) of Genogroup II, genotype 17 (GII.17) NoV using recombinant baculovirus system in insect cells and saliva binding blockade assay to detect their protective potency. Our results showed that GII.17 VLPs could be successfully generated in sf9 insect cells and electron microscopic revealed that GII.17 VLPs was visualized as spherical particles of −35nm in diameter. Immunized mouse with purified VLPs produced GII.17 specific sera and could efficiently block GII.17 VLPs binding to saliva histo-blood group antigens (HBGAs). Together, these results suggested that GII.17 VLPs represent a promising vaccine candidate against NoV GII.17 infection and strongly support further preclinical and clinical studies.