PT  - JOURNAL ARTICLE
AU  - Ling Lian
AU  - Wei He
AU  - Qiuhua Cai
AU  - Hui Zhang
AU  - Chengrong Ren
AU  - Jun Ding
AU  - Huibin Xu
AU  - Yuelong Lin
AU  - Liyan Pan
AU  - Yidong Wei
AU  - Yongsheng Zhu
AU  - Yanmei Zheng
AU  - Linyan Wei
AU  - Hongguang Xie
AU  - Huaan Xie
AU  - Zonghua Wang
AU  - Jianfu Zhang
TI  - Physiological and transcriptome analysis of indica rice ‘MH86’ overexpressing <em>OsSPL14</em>
AID  - 10.1101/496620
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 496620
4099  - http://biorxiv.org/content/early/2018/12/14/496620.short
4100  - http://biorxiv.org/content/early/2018/12/14/496620.full
AB  - OsSPL14, identified as IDEAL PLANT ARCHITECTURE1 (IPA1) or WEALTHY FARMER’S PANICLE (WFP) gene, plays a critical role in regulating rice plant architecture. Here, the study showed that OsSPL14-overexpression transgenic rice plants had shorter growth period, short-narrow flag leaves, and thick-green leaves. Compared with wild type plant ‘MH86’, transgenic plants had higher chlorophyll a (Ca), chlorophyll b (Cb) and carotenoid (Cx) content at both seedling and maturity stage. Meanwhile, transcriptome analysis identified 473 up-regulated and 103 down-regulated genes in transgenic plant. The expression of differentially expressed genes (DEGs) involved in carotenoid biosynthesis, abscisic acid (ABA) metabolism and lignin biosynthesis increased significantly. Most of DEGs participated in “plant hormone signal transduction” and “starch and sucrose metabolism” are also up-regulated in transgenic plant. In addition, there were higher levels of ABA and gibberellin acid (GA3) in OsSPL14-overexpression transgenic plants. Moreover, the content of culm lignin, cellulose, silicon and potassium all increased dramatically. Thus, these results demonstrate that overexpression of OsSPL14 has influence on leaf development, hormone level and culm composition in rice, which provide more insight into understanding the function of OsSPL14.Highlight OsSPL14-overexpression transgenic plants showed shorter growth period, short-narrow flag leaves, and thick-green leaves. Transcript profile, abscisic acid (ABA) and gibberellin acid (GA3) level, and culm composition also changed obviously.AbbreviationsABAabscisic acidDEGsdifferentially expressed genesFCfold ChangeFDRfalse discovery rateGA3gibberellin acidGOgene ontologyJAjasmonate acidKEGGkyotoencyclopedia of genes and genomesqRT-PCRquantitative real-time PCR