TY - JOUR T1 - Wnt/PCP-primed intestinal stem cells directly differentiate into enteroendocrine or Paneth cells JF - bioRxiv DO - 10.1101/2020.08.12.247585 SP - 2020.08.12.247585 AU - Anika Böttcher AU - Maren Büttner AU - Sophie Tritschler AU - Michael Sterr AU - Alexandra Aliluev AU - Lena Oppenländer AU - Ingo Burtscher AU - Steffen Sass AU - Martin Irmler AU - Johannes Beckers AU - Christoph Ziegenhain AU - Wolfgang Enard AU - Andrea C. Schamberger AU - Fien M. Verhamme AU - Oliver Eickelberg AU - Fabian J. Theis AU - Heiko Lickert Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/08/12/2020.08.12.247585.abstract N2 - A detailed understanding of intestinal stem cell (ISC) self-renewal and differentiation is required to better treat chronic intestinal diseases. However, different models of ISC lineage hierarchy1–6 and segregation7–12 are debated. Here we report the identification of Lgr5+ ISCs that express Flattop (Fltp), a Wnt/planar cell polarity (PCP) reporter and effector gene. Lineage labelling revealed that Wnt/PCP-activated Fltp+ ISCs are primed either towards the enteroendocrine or the Paneth cell lineage in vivo. Integration of time-resolved lineage labelling with genome-wide and targeted single-cell gene expression analysis allowed us to delineate the ISC differentiation path into enteroendocrine and Paneth cells at the molecular level. Strikingly, we found that both lineages are directly recruited from ISCs via unipotent transition states, challenging the existence of formerly predicted bi- or multipotent secretory progenitors7–12. Transitory cells that mature into Paneth cells are quiescent and express both stem cell and secretory lineage genes, indicating that these cells are the previously described Lgr5+ labelretaining cells7. Wnt/PCP-activated Lgr5+ ISCs are indistinguishable from Wnt/β-catenin-activated Lgr5+ ISCs based on the expression of stem-cell signature or secretory lineagespecifying genes but possess less self-renewal activity. This suggests that lineage priming and cell-cycle exit is triggered at the post-transcriptional level by polarity cues and a switch from canonical to non-canonical Wnt/PCP signalling. Taken together, we identified the Wnt/PCP pathway as a new niche signal and polarity cue regulating stem cell fate. Active Wnt/PCP signalling represents one of the earliest events in ISC lineage priming towards the Paneth and enteroendocrine cell fate, preceding lateral inhibition and expression of secretory lineagespecifying genes. Thus, our findings provide a better understanding of the niche signals and redefine the mechanisms underlying ISC lineage hierarchy and segregation.Competing Interest StatementF.J.T. reports receiving consulting fees from Roche Diagnostics GmbH and Cellarity Inc., and ownership interest in Cellarity, Inc. and Dermagnostix. S.T. reports receiving consulting fees from Cellarity, Inc. All other authors declare no conflict of interest. ER -