TY - JOUR T1 - CRISPR-Decryptr reveals cis-regulatory elements from noncoding perturbation screens JF - bioRxiv DO - 10.1101/2020.08.13.247007 SP - 2020.08.13.247007 AU - Anders Rasmussen AU - Tarmo Äijö AU - Mariano Ignacio Gabitto AU - Nicholas Carriero AU - Neville Sanjana AU - Jane Skok AU - Richard Bonneau Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/08/14/2020.08.13.247007.abstract N2 - Clustered Regularly Interspace Short Palindromic Repeats (CRISPR)-Cas9 genome editing methods provide the tools necessary to examine phenotypic impacts of targeted perturbations in high-throughput screens. While these technologies have the potential to reveal functional elements with direct therapeutic applications, statistical techniques to analyze noncoding screen data remain limited. We present CRISPR-Decryptr, a computational tool for the analysis of CRISPR noncoding screens. Our method leverages experimental design: accounting for multiple conditions, controls, and replicates to infer the regulatory landscape of noncoding genomic regions. We validate our method on a variety of mutagenesis, CRISPR activation, and CRISPR interference screens, extracting new insights from previously published data.Competing Interest StatementA.R. owns stock in Editas medicine and 10x Genomics. T.A. owns stock in 10x Genomics. R.B. has ongoing or recent consulting or advisory relationships with Eli Lily, Merus, Merck and Epistemic AI. ER -