PT - JOURNAL ARTICLE AU - Fleur Ponton AU - Juliano Morimoto AU - Katie Robinson AU - Sheemal S Kumar AU - Sheena Cotter AU - Kenneth Wilson AU - Stephen J. Simpson TI - Macronutrients modulate resistance to infection and immunity in Drosophila AID - 10.1101/498493 DP - 2018 Jan 01 TA - bioRxiv PG - 498493 4099 - http://biorxiv.org/content/early/2018/12/17/498493.short 4100 - http://biorxiv.org/content/early/2018/12/17/498493.full AB - Immunity and nutrition are two essential modulators of individual fitness However, while the implications of immune function and nutrition on an individual s lifespan and reproduction are known, the interplay between feeding behaviour, infection, and immune function, remains poorly understood. In this study, we used the fruit fly, Drosophila melanogaster, to investigate how infection through septic injury modulates nutritional intake, and how macronutrient balance affects survival to infection by the pathogenic Gram-positive bacterium Micrococcus luteus. Our results show that infected flies maintain carbohydrate intake, but reduce protein intake, thereby shifting from a protein-to-carbohydrate (P:C) ratio of ~1:4 to ~1:10 relative to non-infected and sham-infected flies. Strikingly, we found that the proportion of flies dying after M. luteus infection was significantly lower when flies were fed a low-P high-C diet, revealing that flies shift their macronutrient intake as means of nutritional self-medication against bacterial infection. This is likely due to the effects of macronutrient balance on the regulation of the constitutive expression of innate immune genes, as a low-P high-C diet was linked to an up-regulation in the expression of key antimicrobial peptides. Together, our results reveal the intricate relationship between macronutrient intake and resistance to infection, and integrate the molecular cross-talk between metabolic and immune pathways into the framework of nutritional immunology.