TY - JOUR T1 - A toolkit to generate inducible and interconvertible <em>Drosophila</em> transgenes JF - bioRxiv DO - 10.1101/2020.08.18.256461 SP - 2020.08.18.256461 AU - Franz Wendler AU - Sangbin Park AU - Claire Hill AU - Alessia Galasso AU - Kathleen R. Chang AU - Iman Awan AU - Yulia Sudarikova AU - Mar Bustamante AU - Sichen Liu AU - Ethan Sung AU - Gabrielle Aisabonoko AU - Seung K. Kim AU - Luis Alberto Baena-Lopez Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/08/19/2020.08.18.256461.abstract N2 - The existence of three independent binary systems for conditional gene expression (Gal4/UAS; LexA/LexAop; QF/QUAST) has greatly expanded versatile genetic analyses in the fruit fly Drosophila melanogaster; however, the experimental application of these tools is limited by the need to generate multiple collections of non-interchangeable transgenic fly strains for each inducible gene expression system. To address this practical limitation, we developed a multipurpose modular vector that contains the regulatory elements from all three binary systems, enabling Gal4-, LexA- or QF-dependent expression of transgenes. Our methods also incorporate DNA elements that facilitate independent site-specific recombination and elimination of regulatory UAS, LexAop or QUAST modules with spatial and temporal control, thus offering unprecedented possibilities and logistical advantages for in vivo genetic modulation and efficient interconversion of transgenic fly lines.Competing Interest StatementThe authors have declared no competing interest. ER -