RT Journal Article
SR Electronic
T1 A toolkit to generate inducible and interconvertible <em>Drosophila</em> transgenes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.18.256461
DO 10.1101/2020.08.18.256461
A1 Franz Wendler
A1 Sangbin Park
A1 Claire Hill
A1 Alessia Galasso
A1 Kathleen R. Chang
A1 Iman Awan
A1 Yulia Sudarikova
A1 Mar Bustamante
A1 Sichen Liu
A1 Ethan Sung
A1 Gabrielle Aisabonoko
A1 Seung K. Kim
A1 Luis Alberto Baena-Lopez
YR 2020
UL http://biorxiv.org/content/early/2020/08/19/2020.08.18.256461.abstract
AB The existence of three independent binary systems for conditional gene expression (Gal4/UAS; LexA/LexAop; QF/QUAST) has greatly expanded versatile genetic analyses in the fruit fly Drosophila melanogaster; however, the experimental application of these tools is limited by the need to generate multiple collections of non-interchangeable transgenic fly strains for each inducible gene expression system. To address this practical limitation, we developed a multipurpose modular vector that contains the regulatory elements from all three binary systems, enabling Gal4-, LexA- or QF-dependent expression of transgenes. Our methods also incorporate DNA elements that facilitate independent site-specific recombination and elimination of regulatory UAS, LexAop or QUAST modules with spatial and temporal control, thus offering unprecedented possibilities and logistical advantages for in vivo genetic modulation and efficient interconversion of transgenic fly lines.Competing Interest StatementThe authors have declared no competing interest.