RT Journal Article
SR Electronic
T1 Population genomics of two closely related anhydrobiotic midges reveals differences in adaptation to extreme desiccation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.19.255828
DO 10.1101/2020.08.19.255828
A1 N.M. Shaykhutdinov
A1 G.V. Klink
A1 S.K. Garushyants
A1 O.S. Kozlova
A1 A.V. Cherkasov
A1 T. Kikawada
A1 T. Okuda
A1 D. Pemba
A1 R.M. Deviatiiarov
A1 G.R. Gazizova
A1 A.A. Penin
A1 E.I. Shagimardanova
A1 R. Cornette
A1 O.A. Gusev
A1 G.A. Bazykin
YR 2020
UL http://biorxiv.org/content/early/2020/08/20/2020.08.19.255828.abstract
AB The sleeping chironomid Polypedilum vanderplanki is capable of anhydrobiosis, a striking example of adaptation to extreme desiccation. Tolerance to complete desiccation in this species is associated with the emergence of multiple paralogs of protective genes. One of the gene families highly expressed under anhydrobiosis and involved in this process are protein-L-isoaspartate (D-aspartate) O-methyltransferases (PIMTs). Recently, a closely related anhydrobiotic midge from Malawi, P. pembai, showing the ability to tolerate complete desiccation similar to that of P. vanderplanki, but experiences more frequent desiccation-rehydration cycles due to differences in ecology, was discovered. Here, we sequenced and assembled the genome of P. pembai and performed a population genomics analysis of several populations of P. vanderplanki and a population of P. pembai. We observe positive selection and radical changes in the genetic architecture of the PIMT locus between the two species, including multiple duplication events in the P. pembai lineage. In particular, PIMT-4, the most highly expressed of these PIMTs, is present in six copies in the P. pembai; these copies differ in expression profiles, suggesting possible sub- or neofunctionalization. The nucleotide diversity (π) of the genomic region carrying these new genes is decreased in P. pembai, but not in the orthologous region carrying the ancestral gene in P. vanderplanki, providing evidence for a selective sweep associated with post-duplication adaptation in the former. Overall, our results suggest an extensive recent and likely ongoing, adaptation of the mechanisms of anhydrobiosis.Competing Interest StatementThe authors have declared no competing interest.