RT Journal Article
SR Electronic
T1 Salivary proteins of a gall-inducing aphid and their impact on early gene responses of susceptible and resistant host-plant genotypes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 504613
DO 10.1101/504613
A1 Luis Portillo Lemus
A1 Jessy Tricard
A1 Jérôme Duclercq
A1 Quentin Coulette
A1 David Giron
A1 Christophe Hano
A1 Elisabeth Huguet
A1 Frédéric Lamblin
A1 Anas Cherqui
A1 Aurélien Sallé
YR 2018
UL http://biorxiv.org/content/early/2018/12/22/504613.abstract
AB Plant manipulation by herbivores requires fine-tuned reprogramming of host metabolism, mediated by effector molecules delivered by the parasite into its host. While plant galls may represent the epitome of plant manipulation, secretomes of gall-inducers and their impact on host-plants have been rarely studied. We characterized, with transcriptomic and enzymatic approaches, salivary glands and saliva of a gall-inducing aphid, Phloeomyzus passerinii. Early responses to aphid saliva of plant genes belonging to different metabolic and signaling pathways were assessed in vivo, with poplar protoplasts, and in planta, in a heterologous Arabidopsis system. Several effectors potentially interfering with plant signaling have been identified, including binding proteins, oxidoreductases, and phosphatidylinositol phosphate kinases. Compatible interactions between protoplasts of a susceptible poplar genotype and the saliva of P. passerinii led to an overall downregulation of defense-related genes while an upregulation was observed during both incompatible interactions, with a resistant poplar genotype, and non-host interactions, with the saliva of Myzus persicae, an aphid which does not feed on poplars. Compatible interactions affected both auxin transport and homeostasis potentially leading to an intracellular accumulation of auxin, which was further supported by in planta assays. Our results support the hypothesis that effectors interfere with downstream signaling and phytohormone pathways.