RT Journal Article
SR Electronic
T1 Temporally-Divergent Regulatory Mechanisms Govern Neuronal Development and Diversification in the Neocortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.23.263434
DO 10.1101/2020.08.23.263434
A1 Wen Yuan
A1 Sai Ma
A1 Juliana R. Brown
A1 Kwanho Kim
A1 Vanessa Murek
A1 Lucia Trastulla
A1 Alexander Meissner
A1 Simona Lodato
A1 Ashwin Shetty
A1 Joshua Z. Levin
A1 Jason D. Buenrostro
A1 Michael J. Ziller
A1 Paola Arlotta
YR 2020
UL http://biorxiv.org/content/early/2020/08/24/2020.08.23.263434.abstract
AB Mammalian neocortical neurons span one of the most diverse cell type spectra of any tissue. The regulatory strategies that neurons use during progressive development and maturation remain unclear. We present an integrated single-cell epigenomic and transcriptional analysis of individual classes of neurons from both mouse and marmoset neocortex, sampled during both early postmitotic stages of identity acquisition and later stages of neuronal plasticity and circuit integration. We find that in both species, the regulatory strategies controlling these early and late stages diverge: early postmitotic neurons use molecular regulatory programs with broader tissue distribution and greater evolutionary conservation, while programs active during later neuronal maturation implement more brain- and neuron-specific mechanisms showing greater evolutionary divergence. The data uncovers a temporally-regulated shift in regulatory choices, likely reflecting unique evolutionary constraints on distinct events of neuronal development in the neocortex.Competing Interest StatementThe authors have declared no competing interest.