RT Journal Article
SR Electronic
T1 Human cytomegalovirus genomes sequenced directly from clinical material: variation, multiple-strain infection, recombination and mutation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 505735
DO 10.1101/505735
A1 Nicolás M. Suárez
A1 Gavin S. Wilkie
A1 Elias Hage
A1 Salvatore Camiolo
A1 Marylouisa Holton
A1 Joseph Hughes
A1 Maha Maabar
A1 Vattipally B. Sreenu
A1 Akshay Dhingra
A1 Ursula A. Gompels
A1 Gavin W. G. Wilkinson
A1 Fausto Baldanti
A1 Milena Furione
A1 Daniele Lilleri
A1 Alessia Arossa
A1 Tina Ganzenmueller
A1 Giuseppe Gerna
A1 Petr Hubáček
A1 Thomas F. Schulz
A1 Dana Wolf
A1 Maurizio Zavattoni
A1 Andrew J. Davison
YR 2018
UL http://biorxiv.org/content/early/2018/12/23/505735.abstract
AB The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical material were investigated, focusing on variation, multiple-strain infection, recombination and natural mutation. A total of 207 datasets generated in this and other studies using target enrichment and high-throughput sequencing were analysed, in the process facilitating the determination of genome sequences for 91 strains. Key findings were that (i) it is vital to monitor sequence data quality, especially when analysing intrahost diversity, (ii) intrahost diversity in single-strain infections is much less than that in multiple strain infections, (iii) many recombinant strains have been generated and transmitted during HCMV evolution, and some have survived for thousands of years without further recombination, (iv) mutants lacking gene functions have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae. Future studies in general populations are likely to continue illuminating the evolution, epidemiology and pathogenesis of HCMV.