RT Journal Article SR Electronic T1 PFRED: A computational platform for siRNA and antisense oligonucleotides design JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.08.25.265983 DO 10.1101/2020.08.25.265983 A1 Simone Sciabola A1 Hualin Xi A1 Dario Cruz A1 Qing Cao A1 Christine Lawrence A1 Tianhong Zhang A1 Sergio Rotstein A1 Jason D Hughes A1 Daniel R Caffrey A1 Robert Stanton YR 2020 UL http://biorxiv.org/content/early/2020/08/25/2020.08.25.265983.abstract AB PFRED a software application for the design, analysis, and visualization of antisense oligonucleotides and siRNA is described. The software provides an intuitive user-interface for scientists to design a library of siRNA or antisense oligonucleotides that target a specific gene of interest. Moreover, the tool facilitates the incorporation of various design criteria that have been shown to be important for stability and potency. PFRED has been made available as an open-source project so the code can be easily modified to address the future needs of the oligonucleotide research community. A compiled version is available for downloading at https://github.com/pfred/pfred-gui/releases as a java Jar file. The source code and the links for downloading the precompiled version can be found at https://github.com/pfred.