PT  - JOURNAL ARTICLE
AU  - Alexander Sheh
AU  - Stephen C. Artim
AU  - Monika A. Burns
AU  - Jose Arturo Molina-Mora
AU  - Mary Anne Lee
AU  - JoAnn Dzink-Fox
AU  - Sureshkumar Muthupalani
AU  - James G. Fox
TI  - Common Marmoset Gut Microbiome Profiles in Health and Intestinal Disease
AID  - 10.1101/2020.08.27.268524
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.08.27.268524
4099  - http://biorxiv.org/content/early/2020/08/27/2020.08.27.268524.short
4100  - http://biorxiv.org/content/early/2020/08/27/2020.08.27.268524.full
AB  - Chronic gastrointestinal (GI) diseases are the most common diseases in captive marmosets. The gut microbiome of healthy (n=91), inflammatory bowel disease (IBD) (n=59), and duodenal ulcer/stricture (n=23) captive marmosets was characterized. Healthy marmosets exhibited a “humanized,” Bacteroidetes-dominant microbiome. Despite standardized conditions, cohorts subdivided into Prevotella- and Bacteroides-dominant groups based on marmoset source. IBD was highest in a Prevotella-dominant cohort while strictures were highest in a Bacteroides-dominant cohort. Stricture-associated dysbiosis was characterized by Anaerobiospirillum loss and Clostridium perfringens increases. Stricture tissue presented upregulation of lipid metabolism genes and increased abundance of C. perfringens, a causative agent of GI diseases and intestinal strictures in humans. IBD was associated with a lower Bacteroides:P. copri ratio within each source. Consistent with Prevotella-linked diseases, pro-inflammatory genes were upregulated. This report highlights the humanization of the captive marmoset microbiome and its potential as a “humanized” animal model of C. perfringens-induced enteritis/strictures and P. copri-associated IBD.Competing Interest StatementThe authors have declared no competing interest.