RT Journal Article
SR Electronic
T1 Multi-plane Imaging of Neural Activity From the Mammalian Brain Using a Fast-switching Liquid Crystal Spatial Light Modulator
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 506618
DO 10.1101/506618
A1 Rui Liu
A1 Neil Ball
A1 James Brockill
A1 Leonard Kuan
A1 Daniel Millman
A1 Cassandra White
A1 Arielle Leon
A1 Derric Williams
A1 Shig Nishiwaki
A1 Saskia de Vries
A1 Josh Larkin
A1 David Sullivan
A1 Cliff Slaughterbeck
A1 Colin Farrell
A1 Peter Saggau
YR 2018
UL http://biorxiv.org/content/early/2018/12/30/506618.abstract
AB We report a novel two-photon fluorescence microscope based on a fast-switching liquid crystal spatial light modulator and a pair of galvo-resonant scanners for large-scale recording of neural activity from the mammalian brain. The utilized imaging technique is capable of monitoring large populations of neurons spread across different layers of the neocortex in awake and behaving mice. During each imaging session, all visual stimulus driven somatic activity could be recorded in the same behavior state. We observed heterogeneous response to different types of visual stimuli from ~ 3,300 excitatory neurons reaching from layer II/III to V of the striate cortex.