PT  - JOURNAL ARTICLE
AU  - Marianna Karava
AU  - Peter Gockel
AU  - Johannes Kabisch
TI  - <em>Bacillus subtilis</em> spore surface display of photodecarboxylase for the transformation of lipids to hydrocarbons
AID  - 10.1101/2020.08.30.273821
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.08.30.273821
4099  - http://biorxiv.org/content/early/2020/08/31/2020.08.30.273821.short
4100  - http://biorxiv.org/content/early/2020/08/31/2020.08.30.273821.full
AB  - The display of enzymes on the surface of spores allows the rapid and very simple biotechnological production of immobilized enzymes. Here we describe the development of a Bacillus subtilis spore display platform and its application to produce hydrocarbons from lipids obtained from the oleaginous yeasts Yarrowia lipolytica, Cutaneotrichosporon oleaginosus as well as olive oil.Lipid hydrolysis was examined in a bienzymatic one-pot cascade using a commercially immobilized lipase (RO lipase) as well as spores with and without additional heterologous lipase expression. Decarboxylation of the released fatty acids was achieved displaying a photodecarboxylase (CvFAP) on the spore surface. Differences in composition of the formed hydrocarbons were observed depending on the lipids source. Using 3D printed lighting equipment titers of up to 64.0 ± 5.6 mg/L hydrocarbons were produced.Competing Interest StatementThe authors have declared no competing interest.AbbreviationsSDWspore dry weightTAGstriacylglyceridesHChydrocarbonCo. oilnatural oil produced by Cutaneotrichosporon oleaginosusYali. oilnatural oil produced by Yarrowia lipolyticaRORhizopus oryzaeWOwithoutSGSchaffers glucose