TY - JOUR T1 - White matter integrity requires continuous myelin synthesis at the inner tongue JF - bioRxiv DO - 10.1101/2020.09.02.279612 SP - 2020.09.02.279612 AU - Martin Meschkat AU - Anna M. Steyer AU - Marie-Theres Weil AU - Kathrin Kusch AU - Olaf Jahn AU - Lars Piepkorn AU - Paola Agüi-Gonzalez AU - Nhu Thi Ngoc Phan AU - Torben Ruhwedel AU - Boguslawa Sadowski AU - Silvio O. Rizzoli AU - Hauke B. Werner AU - Hannelore Ehrenreich AU - Klaus-Armin Nave AU - Wiebke Möbius Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/09/03/2020.09.02.279612.abstract N2 - Myelin, the electrically insulating axonal sheath, is composed of lipids and proteins with exceptionally long lifetime. This raises the question how myelin function is affected by myelin turnover. We have studied the integrity of myelinated tracts after experimentally preventing the formation of new myelin in the CNS of adult mice, using an inducible Mbp null allele. Oligodendrocytes survived recombination, continued expressing myelin genes, but failed to maintain compacted myelin sheaths. Using 3D electron microscopy and mass spectrometry imaging we visualized myelin-like membranes that failed to incorporate adaxonally, most prominently at juxta-paranodes. Myelinoid body formation indicated degradation of existing myelin at the abaxonal side and at the inner tongue of the sheath. Compacted myelin thinning and shortening of internodes, with about 50% myelin lost after 20 weeks (=5 months), ultimately led to axonal pathology and neurological disease. These data reveal that functional axon-myelin units require the continuous incorporation of new myelin membranes.Competing Interest StatementThe authors have declared no competing interest. ER -