RT Journal Article
SR Electronic
T1 Buckling of epithelium growing under spherical confinement
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 513119
DO 10.1101/513119
A1 Anastasiya Trushko
A1 Ilaria Di Meglio
A1 Aziza Merzouki
A1 Carles Blanch-Mercader
A1 Shada Abuhattum
A1 Jochen Guck
A1 Kevin Alessandri
A1 Pierre Nassoy
A1 Karsten Kruse
A1 Bastien Chopard
A1 Aurélien Roux
YR 2019
UL http://biorxiv.org/content/early/2019/01/07/513119.abstract
AB Many organs, such as the gut or the spine are formed through folding of an epithelium. Whereas genetic regulation of epithelium folding has been investigated extensively, the nature of the mechanical forces driving this process remain largely unknown. Here we show that monolayers of identical cells proliferating on the inner surface of elastic spherical shells can spontaneously fold. By measuring the elastic deformation of the shell we inferred the forces acting within the monolayer. Using analytical and numerical theories at different scales, we found that the compressive stresses arising within the cell monolayer through proliferation quantitatively account for the shape of folds observed in experiments. Our study shows that forces arising from epithelium growth are sufficient to drive folding by buckling.One Sentence Summary Epithelial cells proliferating in an artificial, elastic hollow sphere accumulate compressive stresses that drive inward buckling.