PT  - JOURNAL ARTICLE
AU  - Dattani, Justine
AU  - Barahona, Mauricio
TI  - Stochastic models of gene transcription with upstream drives: exact solution and sample path characterization
AID  - 10.1101/055202
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 055202
4099  - http://biorxiv.org/content/early/2016/05/24/055202.short
4100  - http://biorxiv.org/content/early/2016/05/24/055202.full
AB  - Gene transcription is a highly stochastic and dynamic process. As a result, the mRNA copy number of a given gene is heterogeneous both between cells and across time. We present a framework to model gene transcription in populations of cells with time-varying (stochastic or deterministic) transcription and degradation rates. Such rates can be understood as upstream cellular drives representing the effect of different aspects of the cellular environment. We show that the full solution of the master equation contains two components: a model-specific, upstream effective drive, which encapsulates the effect of the cellular drives (e.g., entrainment, periodicity or promoter randomness), and a downstream transcriptional Poissonian part, which is common to all models. Our analytical framework allows us to treat cell-to-cell and dynamic variability consistently, unifying several approaches in the literature. We apply the obtained solution to characterize several gene transcription models of experimental relevance, and to explain the influence on gene transcription of synchrony, stationarity, ergodicity, as well as the effect of time-scales and other dynamic characteristics of drives. We also show how the solution can be applied to the analysis of single-cell data, and to reduce the computational cost of sampling solutions via stochastic simulation.