RT Journal Article
SR Electronic
T1 Otx2 signals from the choroid plexus to regulate adult neurogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 243659
DO 10.1101/243659
A1 Planques, Anabelle
A1 Oliveira Moreira, Vanessa
A1 Dubreuil, Chantal
A1 Prochiantz, Alain
A1 Di Nardo, Ariel A
YR 2019
UL http://biorxiv.org/content/early/2019/01/08/243659.abstract
AB Proliferation and migration during adult neurogenesis are regulated by a microenvironment of signaling molecules originating from local vasculature, from cerebrospinal fluid produced by the choroid plexus, and from local supporting cells including astrocytes. Here, we focus on the function of OTX2 homeoprotein transcription factor in the mouse adult ventricular- subventricular zone (V-SVZ) which generates olfactory bulb neurons. We find that OTX2 secreted by choroid plexus is transferred to supporting cells of the V-SVZ and rostral migratory stream. Deletion of Otx2 in choroid plexus affects neuroblast migration and reduces the number of olfactory bulb newborn neurons. Adult neurogenesis was also decreased by expressing secreted single-chain antibodies to sequester OTX2 in the cerebrospinal fluid, demonstrating the importance of non-cell autonomous OTX2. We show that OTX2 activity modifies extracellular matrix components and signaling molecules produced by supporting astrocytes. Thus, we reveal a multi-level and non-cell autonomous role of a homeoprotein and reinforce the choroid plexus and astrocytes as key niche compartments affecting adult neurogenesis.