RT Journal Article
SR Electronic
T1 Impact of chronic transcranial Random-Noise Stimulation (tRNS) on prefrontal cortex excitation-inhibition balance in juvenile mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.04.282889
DO 10.1101/2020.09.04.282889
A1 Carlos A. Sánchez-León
A1 Álvaro Sánchez-López
A1 María A. Gómez-Climent
A1 Isabel Cordones
A1 Roi Cohen Kadosh
A1 Javier Márquez-Ruiz
YR 2020
UL http://biorxiv.org/content/early/2020/09/05/2020.09.04.282889.abstract
AB Transcranial random noise stimulation (tRNS), a non-invasive neuromodulatory technique capable of altering cortical activity, has been proposed to improve the signal-to-noise ratio at the neuronal level and the sensitivity of the neurons following an inverted U-function. The aim of this study was to examine the effects of tRNS on vGLUT1 and GAD 65-67 and its safety in terms of pathological changes. For that, juvenile mice were randomly distributed in three different groups: “tRNS 1x” receiving tRNS at the density current used in humans (0.3 A/m2, 20 min), “tRNS 100x” receiving tRNS at two orders of magnitude higher (30.0 A/m2, 20 min) and “sham” (0.3 A/m2, 15 s). Nine tRNS sessions during five weeks were administered to the prefrontal cortex of alert animals. No detectable tissue macroscopic lesions were observed after tRNS sessions. Post-stimulation immunohistochemical analysis of GAD 65-67 and vGLUT1 immunoreactivity showed a reduced GAD 65-67 immunoreactivity levels in the region directly beneath the electrode for tRNS 1x group with no significant effects in the tRNS 100x nor sham group. The observed results points to an excitatory effect associated with a decrease in GABA levels in absence of major histopathological alterations providing a novel mechanistic explanation for tRNS effects.Competing Interest StatementThe authors have declared no competing interest.