PT  - JOURNAL ARTICLE
AU  - Andrew C Symonds
AU  - Clare E Buckley
AU  - Charlotte A Williams
AU  - Jonathan DW Clarke
TI  - Coordinated assembly and release of adhesions builds apical junctional belts during <em>de novo</em> polarisation of an epithelial tube
AID  - 10.1101/2020.02.16.950543
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.16.950543
4099  - http://biorxiv.org/content/early/2020/09/07/2020.02.16.950543.short
4100  - http://biorxiv.org/content/early/2020/09/07/2020.02.16.950543.full
AB  - Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that NOK/Pals1/Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Nok and Rab11a mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose these roles for both Nok and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction remodelling role of Nok is not specific to dividing cells.Competing Interest StatementThe authors have declared no competing interest.