RT Journal Article
SR Electronic
T1 Seasonal and long-term consequences of esca on grapevine stem xylem integrity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.07.282582
DO 10.1101/2020.09.07.282582
A1 G. Bortolami
A1 E. Farolfi
A1 E. Badel
A1 R. Burlett
A1 H. Cochard
A1 N. Ferrer
A1 A. King
A1 L.J. Lamarque
A1 P. Lecomte
A1 M. Marchesseau-Marchal
A1 J. Pouzoulet
A1 J.M. Torres-Ruiz
A1 S. Trueba
A1 S. Delzon
A1 G.A. Gambetta
A1 C.E.L. Delmas
YR 2020
UL http://biorxiv.org/content/early/2020/09/08/2020.09.07.282582.abstract
AB Hydraulic failure has been extensively studied during drought-induced plant dieback, but its role in plant-pathogen interactions is under debate. During esca, a grapevine (Vitis vinifera) disease, symptomatic leaves are prone to irreversible hydraulic dysfunctions but little is known about the hydraulic integrity of perennial organs over the short- and long-term.We investigated the effects of esca on stem hydraulic integrity in naturally infected plants within a single season and across season(s). We coupled in vivo X-ray microtomography visualizations with direct (ks) and indirect (kth) hydraulic conductivity measurements, and tylose and vascular pathogen detection.Although no loss of hydraulic conductivity (PLC) was observed in asymptomatic and 40% of symptomatic stems, 60% of symptomatic stems presented xylem occlusions with subsequent PLC, which could reach critical levels (over 50% PLC). A loss of stem ks was observed simultaneously, or after, the occurrence of leaf symptoms in the presence of tyloses. The impact of esca on xylem integrity was only seasonal and no long-term impact of disease history was recorded.Our study demonstrated how and to what extent a vascular disease such as esca, affecting xylem integrity, could amplify plant mortality by hydraulic failure.Competing Interest StatementThe authors have declared no competing interest.