RT Journal Article
SR Electronic
T1 Targeting of RBM10 to S1-1 Nuclear Bodies: Targeting Sequences and its Biological Significance
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 516831
DO 10.1101/516831
A1 Ling-Yu Wang
A1 Sheng-Jun Xiao
A1 Hiroyuki Kunimoto
A1 Kazuaki Tokunaga
A1 Hirotada Kojima
A1 Masatsugu Kimura
A1 Takahiro Yamamoto
A1 Naoki Yamamoto
A1 Zhao Hong
A1 Koji Nishio
A1 Hideo Yamane
A1 Tokio Tani
A1 Koichi Nakajima
A1 Hiroyoshi Iguchi
A1 Akira Inoue
YR 2019
UL http://biorxiv.org/content/early/2019/01/10/516831.abstract
AB RBM10 is an RNA-binding protein that regulates alternative splicing (AS). This protein localizes to the extra-nucleolar nucleoplasm and S1-1 nuclear bodies (NBs). We investigated the biological significance of RBM10 localization to S1-1 NBs, which is poorly understood. Our analyses revealed that RBM10 possesses two S1-1 NB-targeting sequences (NBTSs), one in the KEKE motif region and another in the C2H2 Zn finger (ZnF). These NBTSs acted synergistically and were sufficient for localization of RBM10 to S1-1 NBs. Furthermore, the C2H2 ZnF not only acted as an NBTS, but was also essential for regulation of AS by RBM10. RBM10 did not participate in S1-1 NB formation. We confirmed the previous finding that localization of RBM10 to S1-1 NBs increases as cellular transcriptional activity decreases and vice versa. These results indicate that RBM10 is a transient component of S1-1 NBs and is sequestered in these structures via its NBTSs when cellular transcription decreases. We propose that the NB-targeting activity of the C2H2 ZnF is induced when it is not bound to pre-mRNA or the splicing machinery complex under conditions of reduced transcription.