RT Journal Article
SR Electronic
T1 Peripheral microtubules ensure asymmetric furrow positioning in neural stem cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.10.291112
DO 10.1101/2020.09.10.291112
A1 Alexandre Thomas
A1 Emmanuel Gallaud
A1 Aude Pascal
A1 Laurence Serre
A1 Isabelle Arnal
A1 Laurent Richard-Parpaillon
A1 Matthew Scott Savoian
A1 Régis Giet
YR 2020
UL http://biorxiv.org/content/early/2020/09/11/2020.09.10.291112.abstract
AB Neuroblast (NB) cell division is characterized by a basal positioning of the cleavage furrow resulting in a large difference in size between the future daughter cells. In animal cells, furrow placement and assembly is governed by centralspindlin, a highly conserved complex that accumulates at the equatorial cell cortex of the future cleavage site and at the spindle midzone. In contrast to model systems studied so far, these two centralspindlin populations are spatially and temporally separated in NBs. A cortical leading pool is located at the basal cleavage furrow site and a second pool accumulates at the midzone before travelling to the site of the basal cleavage furrow during cytokinesis completion. By manipulating microtubule (MT) dynamics, we show that the cortical centralspindlin population requires peripheral astral microtubules and the Chromosome Passenger Complex (CPC) for efficient recruitment. Loss of this pool does not prevent cytokinesis but enhances centralspindlin levels at the midzone leading to furrow repositioning towards the equator and decreased size asymmetry between daughter cells. Together these data reveal that the asymmetrical furrow placement characteristic of NBs results from a competition between spatially and functionally separate centralspindlin pools in which the cortical pool is dominant and requires peripheral astral microtubules.Competing Interest StatementThe authors have declared no competing interest.