TY - JOUR T1 - Cdk1 in germline and the dose of somatic EGFR signaling determine the rate of transit amplification JF - bioRxiv DO - 10.1101/521336 SP - 521336 AU - Purna Gadre AU - Shambhabi Chatterjee AU - Bhavna Varshney AU - Chetanchandra Joshi AU - Samir Gupta AU - Nitin Nitsure AU - Krishanu Ray Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/01/15/521336.abstract N2 - Stem cell progeny undergoes a series of mitoses, called transit amplifying (TA) divisions, before terminal differentiation. To quantify the impact of the intrinsic and extrinsic factors regulating the rates of TA divisions, we developed a simplified mathematical model which could predict the periods of the germline divisions from the stage-wise distributions of spermatogonial cysts in Drosophila testis. Analysis of the wild-type data using this model suggested that the cell cycle periods speed up after the second TA division due to shortening of the G1 phases. Further, suppression of the cell cycle checkpoint control in germline and upregulation of the somatic EGFR signaling is estimated to slow-down the rates of both the germline stem cell and TA divisions, increasing the cellular life spans at each stage. Together, these results suggest that higher levels of EGFR activation could attenuate cell division rates in the neighborhood, controlling the extents of tissue growth.HighlightsGSC progeny continues to divide at the same rate for two successive cycles.Subsequent transition to transit-amplifying mode speeds up the proliferation rates.Cdk1 expression in the germline cells controls the rates of GSC and TA divisions.EGFR activation level in the somatic cyst cells determines the rate of TA divisions. ER -